Rio's state court opened an inquiry in March 2016 into whether the mayor had granted "excessive" and "unjustified" benefits to Fiori Empreendimentos, which never paid an environmental impact fee of 1.8 million reais ($533,000).
Mayor Eduardo Paes said he would appeal the court ruling, which saw up to 181,000 reais of his assets frozen.
"Contrary to what the prosecution claims, the city council of Rio demanded... that Fiori Empreendimentos Imobiliarios pay the tax so that authorization could be granted for it to remove exotic vegetation" on the nature reserve where the course was built, the mayor's office said in a press release, providing documents supporting its claims.
However, the company has never paid the tax.
The golf course, which cost $19 million, was constructed in the city's west. There are two other private golf courses in Rio which did not meet standards to host Olympic golf competitions.
Friday's court ruling specifies that Paes and the company carry out an auction of assets to pay off the sum.
Paes' term in office concludes at the end of the year. He will be replaced by Marcelo Crivella from the socially conservative Brazilian Republican Party (PRB), who won the mayoral race in October municipal elections.
Published : December 10, 2016
By : Agence France-Presse Rio de Janeiro