Revenue rose by 114 per cent to 316.2 million dollars, according to a company statement.
Despite the loss chief executive Dick Costolo said Twitter "had another very strong financial quarter."
"I'm confident in our ability to build the largest daily audience in the world," he added.
The number of active monthly users increased by 23 per cent to 284million, the statement said.
Twitter shares fell about 10 per cent in extended trading following the announcement.