California-based eBay unveiled the move even as it announced its profit in the fourth quarter rose to $936 million on $4.9 billion in revenue.
The job cuts will be across eBay's three divisions: Marketplaces, Enterprise and PayPal.
"We are on the right strategic path, and we are acting decisively and aggressively as we position eBay and PayPal for success," eBay CEO John Donahoe said.
The reorganisation will return eBay to its roots with the "Marketplaces" division, which includes its auctions and online retail sales and accounted for nearly half its 2014 revenues.
Donahoe said the job cuts were necessary "to simplify the organisation, reduce complexity, speed decision making and create competitive cost structures."
eBay announced plans last year to spin off PayPal amid pressure from activist shareholder Carl Icahn, and said the move would help the unit compete better in the fast-moving online payments segment.
In a further move to refocus, eBay said it would likely also shed its Enterprise division, which creates online sites for traditional retailers, in a sale or public offering to create an independent company.
"Enterprise is a strong business and a leading partner for large retailers, managing mission-critical components of their e-commerce initiatives," a statement said.
"However, it has become clear that it has limited synergies with either business and a separation will allow both to focus exclusively on their core markets, as we create two independent world-class companies."
eBay said it reached a "standstill agreement" with Icahn that calls for Icahn Capital executive Jonathan Christodoro to be named to eBay's board.
Icahn said in a separate statement that Christodoro would "have the ability to transition to PayPal's board once the spinoff occurs."
The deal also includes limits on any "poison pill" for PayPal that could block a proposed buyout.
"PayPal's charter documents will contain a number of corporate governance provisions that we suggested and which we believe will greatly enhance shareholder value at PayPal," Icahn said.