
A 59-year-old man died in the town of Louding on Monday, after coming into contact with live poultry, state-run news agency Xinhua reported.
It was the first fatality from the disease in the south-eastern province of Hunan.
On Sunday, a woman died in Foshan, in the central province of Guangdong. A total of 113 cases have been reported across the country this year.
Concerns have been raised as many of China's 1.3-billion population travel at the end of the new year holiday this week.
Authorities have advised people to contact hospitals if they develop a fever after contact with live poultry, but experts say transmission of the disease between humans remains unlikely, the report said.
DPA