The final flight took off from Nakhon Ratchasima (Korat) airport at 7am on Tuesday with just 10 passengers on board.
Closure of the route comes after Nakhon Ratchasima’s governor called a September 16 meeting with Nok Air CEO Wutthiphum Jurangkool but failed to persuade him to continue the service.
The low-cost carrier launched its first flight between Nakhon Ratchasima and Chiang Mai on August 2, using 86-seater Bombardier Q400 NextGen turboprop planes.
However, the service fell short of its target for commercial viability, with some flights carrying just nine passengers.
Governor Wichian Janthornnothai now wants other Nok Air services to detour to Korat, including the Bangkok-Chiang Mai flight.