Twitter users expressed dismay over the company's alleged plan to order tweets based on a relevance-focused algorithm rather than chronologically - changes which the news website BuzzFeed reported could happen as early as this week.
In response to the resulting RIP Twitter hashtag, which was trending on the social media site at the weekend, the company's chief executive Jack Dorsey denied the report.
"We love the live stream," he wrote in a tweet, adding that the company is working to "refine" its service.
Investors have been sceptical about Twitter's prospects for months.
The company's stocks cost more than 50 dollars in April.
However, they began to decline as user numbers stagnated.
Twitter is set to release its latest earnings report on Wednesday.
- DPA